|
週次 |
日期 |
單元主題 |
|
第1週 |
09/13 |
（I）Introduction
（II）DNA structure and function
(1)DNA as store of information |
|
第1週 |
09/15 |
(A) Outline of major experiments that led to the acceptance of DNA as the genetic material
(B) Structure of nucleic acid, base pairing, tautomeric shift, DNA-A,-B,-Z forms
(C) Denaturing and renaturing of DNA
(D) Supercoiled DNA and topoisomerase |
|
第2週 |
09/20 |
(2) DNA technology, genomic size and genetic content, isolation of DNA by centrifugation and electrophoresis
(3) Replication of DNA |
|
第2週 |
09/22 |
(A) The replicon: unit of replication |
|
第3週 |
09/27 |
(4) Genome: organization and evolution |
|
第3週 |
09/29 |
(B) The apparatus for DNA replication
(C) Telomere |
|
第4週 |
10/04 |
(5) Extrachromosome DNA, nucleosome, chromosome and chromatid |
|
第4週 |
10/06 |
(6) The dynamic genome
(A) Recombination and transposition of DNA in the prokaryotic genome
(B) Rearrangement and amplification in the eukaryotic genome
|
|
第5週 |
10/11 |
(4) DNA damage and mutation |
|
第5週 |
10/13 |
(5) DNA repair and modification |
|
第6週 |
10/18 |
第一次期中考(考試範圍0913-10/13) |
|
第6週 |
10/20 |
(A) Transcriptional Regulation in Eukaryotic Chromatin I |
|
第7週 |
10/25 |
(B) Transcriptional Regulation in Eukaryotic Chromatin II |
|
第7週 |
10/27 |
(2) Transcription and regulation (eukaryotic RNA pol. I and III) |
|
第8週 |
11/01 |
(5) DNA repair and modification |
|
第8週 |
11/03 |
（IV）Translation
(1) Assembly line for protein synthesis
(2) Transfer RNA is the translated adaptor |
|
第9週 |
11/08 |
(3) Ribosomes provide a translation factory
(4) Codons and their significance
(5) Translation in cell free system(投影片與上一次上課相同) |
|
第9週 |
11/10 |
(6) Post-translational modification
I. Structure
II. Mechanism and protein degradation(吳君泰老師無投影片檔案) |
|
第10週 |
11/17 |
（V）Recombinant DNA technology（I）
(1) Cloning of DNA：transformation and screening of transformants
(2) Construction of cDNA and genomic libraries
(3) Colony and plaque hybridization, Southern blot, Northern blot, Western blot analysis
(4) Primer extension, S1 nuclease mapping and RNase protection assay
(5) Differential display |
|
第11週 |
11/24 |
(9) Phage strategies : control of lytic and lysogenic pathway
(10) Phage display library
(11) A brief introduction to animal viruses(吳漢中老師,楊宏志老師無投影片檔案) |
|
第11週 |
11/22 |
(6) Site-directed mutagenesis and polymerase chain reaction
(7) Application of restriction fragment length polymorphism and variable number of tandem repeat for genetic disease analysis
(8) Genome-wide sequencing strategies |
|
第12週 |
11/29 |
第二次期中考(範圍10/20-11/29) |
|
第12週 |
12/01 |
張富雄（VI）Construction of the cells
(1) Signal transduction in prokaryotes
(2) Signal transduction in eukaryotes |
|
第13週 |
12/06 |
許秉寧（VII）Genes in the development
(1) Immune diversity and immunotherapy |
|
第13週 |
12/08 |
張百恩(2) Gene regulation and development, RNA interference, micro RNA |
|
第14週 |
12/13 |
葉秀慧(3) Oncogenes
李明學(4) Tumor suppressor genes |
|
第14週 |
12/15 |
（IX）Recombinant DNA technology（II）
董馨蓮(1) Expression of cloned gene in prokaryotic and eukaryotic cells
詹世鵬(2) Yeast genetics and its applications |
|
第15週 |
12/20 |
錢宗良(5) Transgenic techniquesresearch |
|
第15週 |
12/22 |
俞松良(4) Current development of genome projects and their impacts to human diseases |
|
第16週 |
12/27 |
余明俊(3) Applications of bioinformatics and genomics in biomedical |
|
第16週 |
12/29 |
胡務亮(6) Gene therapy |
|
第17週 |
1/03 |
期末考(範圍12/01-12/29) |